Department of Biology, Ave Maria University

BIOL 401: Microbiology with lab, Spring 2008

I.

COURSE:

BIOL401

PREREQUISITES:

BIOL211, BIOL212

CREDIT HOURS:

4

PROFESSOR: 

Dr. Stephen Cronin

OFFICE HOURS:

Monday 12:10pm-1:50pm, Thursday 1pm-1:50pm, Academic Building Room 3051

PHONE:

(239) 280-1617

E-MAIL:  

stephen.cronin@avemaria.edu

SEMESTER:

Spring 2008

TIME AND PLACE:

Monday, Wednesday and Friday 2:10pm to 3:10pm, Tuesday 1:00pm-4:00pm in Academic Building Room 2032

 

II.      COURSE DESCRIPTION:

 

BIOL401 is general study of microorganisms (bacteria and fungi), emphasizing morphology, physiology, ecological relationships, and the nature of disease and its control.  Consideration is also given to viruses.  The laboratory portion of the class is a general study of microbiological techniques and applications.

 

III.    COURSE OBJECTIVE

At the end of this course, students should be able 1. Grow, maintain and identify microbial cultures using standard techniques.  2. Draw conclusions about the physiology of microorganisms from the results of laboratory tests.  3. Communicate accurately and intelligibly general knowledge about microbial physiology, morphology, ecology and role in disease transmission. 4. Research, understand, and evaluate current literature in microbiology at a basic level.

 

IV.    REQUIRED COURSEWORK:

1.  Tests will consist of questions that need to be answered in paragraph format.

2.  Final exam The final exam will be similar to the in class tests but will be longer.  The final will be cumulative.

3.  Lab assignments are take-home assignments relating to laboratory exercises.

4.  Current microbiology assignment is a written review of a current paper in microbiology.

 

V.      EVALUATION PROCESS

The tests, and final exam will evaluate students’ ability to communicate general knowledge about microbial physiology, morphology, ecology and role in disease transmission. Lab assignments will assess the students’ ability to carry out common laboratory procedures in microbiology, and draw conclusions from the results of those procedures. The current microbiology assignment will assess the facility of students to research understand and evaluate current literature in microbiology.

 

VI.    GRADING

Your grade for the lecture portion of BIOL401 will be worth 50% of the total grade.  The grade for the laboratory portion will count for the other 50% of the class.  However, to pass BIOL401, you must have a passing grade in both the lecture and the laboratory sections of the class.  Lecture and laboratory sections will be graded out of 200pts.

1. Five in class tests                                     25%   100pts

2. Comprehensive final                                 25%   100pts

3. Laboratory assignments                            40%   160pts

4. Current microbiology                               20%     40pts

 

In order for you to receive credit for work: 

1.     You must do the work.  Unless otherwise instructed, tests and exams will be taken individually.  No one else may take test or exams for you.  Representing the work of others as your own is plagiarism.  You should seriously read the section of the student handbook on plagiarism.

2.     You must do the work on time.  Tests and exams must be taken during the scheduled time.  If a test is missed due to illness, you must take the test as soon as possible afterward.  Assignments must be handed in at the assigned time.  Tests missed for unauthorized reasons may be taken for ˝ credit.  Assignments turned in late will lose 10% of the grade for each day the assignment is late.

3.      You must do the work using only authorized material, communication, aid and assistance.  If you are unsure about whether assistance is authorized, you should ask.  Students are expected uphold the honor code. 

 

VII.   REQUIRED TEXTS:

 

Willey, Sherwood, and Woolverton, Microbiology, 7th ed. 2008 McGraw Hill, ISBN 978-0-070299291-5

Harley Laboratory Exercises in Microbiology 7th ed. 2008 McGraw Hill, ISBN 978-0-07-299293-9

 

 

VIII. SCHEDULE:

 

January

14

Introduction Chapter 1 in Willey et al.

 

15 Lab

 

 

16

Prokaryotic Cell Structure Chapter 3 in Willey et al.

 

18

Prokaryotic Cell Structure Chapter 3 in Willey et al.

 

21

Last Day to Add Microbial Nutrition Chapter 5 in Willey et al.

 

22 Lab

Dilutions, Microscopy EX 1, 2, 3, 4, 5 in Harley, Chapter 2 sections 2.1, 2.2 in Willey et al.

 

23

Microbial Nutrition Chapter 5 in Willey et al.

 

25

TEST1

 

28

Last Day to Drop Microbial Growth Chapter 6 sections 6.1, 6.2in Willey et al.

 

29 Lab

Culturing Bacteria, Lab Ex 13, 14, 15, 16, 17 in Harley, Chapter 5 sections 5.7, 5.8 in Willey et al.

 

30

Microbial Growth Chapter 6, sections 6.5, 6.6

February

1

Control of Microorganisms Chapter 7

 

4

Metabolism of Microbes Chapter 8 sections 8.1-8.6

 

5 Lab

Staining Techniques EX 6, 7, 8, 10 in Harley et al. Chapter 2, section 2.3 in Willey et al.

 

6

Ash Wednesday Metabolism of Microbes Chapter 8 sections 8.6-8.10

 

8

Catabolism Willey et al. chapter 9 sections 9.1-9.4

 

11

Catabolism Willey et al.chapter 9 sections 9.4-9.8

 

12 lab

Oxygen and determining bacterial numbers EX 17, 18,19 in Harley, Chapter 9 sections 9.6 and 9.7 in Willey et al.

 

13

Catabolism Willey et al.chapter 9 sections 9.8-9.12

 

15

TEST2

 

18

Last Day to Withdraw Without Penalty Biosynthesis Willey chapter 10 sections 10.1 to 10.4

 

19 lab

Carbohydrate & Lipid Metabolism EX 20,21,22 in Harley

 

20

Biosynthesis Willey chapter 10 sections 10.4 to 10.7

 

22

Viruses Willey chapter 16 sections 16.1 to 16.4

 

25

Viruses Willey chapter 16 sections 16.4 to 16.7

 

26 lab

Carbohydrate & Lipid Metabolism Day 2 EX 20,21,22 in Harley

Protein Metabolism EX 25-34 in Harley

 

27

Bacteriophage Willey chapter 17 sections 17.1 to 17.3

 

29

Bacteriophage Willey chapter 17 sections 17.4 to 17.6

March

3

Midterm reading day, No class

 

4 lab

Protein Metabolism Day 2 EX 25-34 in Harley

Bacterial Genetics EX 61, 62, 63 in Harley

 

5

Eukaryotic Viruses Willey chapter 18 sections 18.1-18.2

 

7

Eukaryotic Viruses Willey chapter 18 sections 18.3-18.5

 

10

TEST3

 

11 lab

Bacterial Genetics Day 2 EX 61, 62, 63 in Harley

 

 

12

Innate Immune Responses Willey chapter 31 sections 31.1-31.4

 

14

Innate Immune Responses Willey chapter 31 sections 31.5-31.6

 

17

Adaptive Immunity Willey chapter 32 sections 32.1-32.4

 

18 lab

 

 

19

Adaptive Immunity Willey chapter 32 sections 32.5-32.7

 

21

Good Friday, No Classes

 

24

Easter week, No Classes

 

25

Easter week, No Classes

 

26

Easter week, No Classes

 

28

Easter week, No Classes

 

31

Adaptive Immunity Willey chapter 32 sections 32.8-32.11

April

1 lab

Effects of Environmental Factors on Growth EX 39, 40, 41 in Harley

 

2

 

 

4

TEST4

 

7

 

 

8 lab

Effects of Environmental Factors on Growth EX 39, 40, 41, 42, 43, 44 in Harley

 

9

Pathogenicity Willey chapter 33 sections 33.1-33.4

 

11

Pathogenicity Willey chapter 33 sections 33.4-33.6

 

14

Antimicrobial Chemotherapy Willey chapter 34 sections 34.1-34.5

 

15 lab

Effects of Environmental Factors on Growth EX 42, 43, 44 in Harley

 

16

Antimicrobial Chemotherapy Willey chapter 34 sections 34.6-34.9

 

18

Epidemiology Willey chapter 36 sections 36.1-36.5

 

21

Epidemiology Willey chapter 36 sections 36.6-36.11

 

22 lab

Determination of Bacterial Growth Rate  EX 45 in Harley

 

23

 

 

25

TEST 5

 

28

 

 

29 lab

 

 

30

 

May

1

Reading day

 

2

Reading day

 

3-8

Final exams